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lobind deep well plate  (Eppendorf AG)


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    Structured Review

    Eppendorf AG lobind deep well plate
    Lobind Deep Well Plate, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 95/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lobind+deep+well+plate/Deepwell+Plate/pmc13083930-528-12-16
    Average 95 stars, based on 208 article reviews
    lobind deep well plate - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Mass Spectrometry:

    Article Title: Expanding the toolbox to develop IAP-based degraders of TEAD transcription factors.
    Article Snippet: .. Protein lysates (20 μg protein per replicate) were transferred to 0.5 mL LoBind Deep Well plate (Eppendorf) and prepared for mass spectrometry analysis using the USP3 protocol91. ..

    Article Title: Expanding the toolbox to develop IAP-based degraders of TEAD transcription factors
    Article Snippet: .. Protein lysates (20 μg protein per replicate) were transferred to 0.5 mL LoBind Deep Well plate (Eppendorf) and prepared for mass spectrometry analysis using the USP3 protocol . .. Briefly, samples were subjected to simultaneous reduction and alkylation with a final concentration of 10 mM Tris (2-carboxyethyl) phosphine (TCEP) and 40 mM 2-chloracetamide followed by heating at 95 °C for 10 min. Prewashed magnetic PureCube Carboxy agarose beads (20 μl, Cube Biotech) were added to all the samples along with acetonitrile (ACN, 70% v/v final concentration) and incubated at room temperature for 20 min.

    Article Title: Functional characterisation of components in two Plasmodium falciparum Cullin-RING-Ligase complexes
    Article Snippet: .. Cell lysates (20 μg protein per replicate) were transferred to 0.5 ml LoBind Deep Well plate (Eppendorf) prepared for mass spectrometry analysis using the modified SP3 protocol with some modifications. .. Briefly, samples were subjected to simultaneous reduction and alkylation with a final concentration of 10 mM Tris (2-carboxyethyl) phosphine (TCEP) and 40 mM 2-chloracetamide followed by heating at 95 °C for 10 min. Prewashed magnetic PureCube Carboxy agarose beads (20 μl, Cube Biotech) were added to all the samples along with acetonitrile (ACN,70% v/v final concentration) and incubated at room temperature for 20 min.

    Article Title: Caspase-2 deficiency drives pathogenic liver polyploidy and increases age-associated hepatocellular carcinoma in mice
    Article Snippet: Tissue lysates were sonicated and cleared by centrifugation at 20,000 g (4°C), and protein concentration was determined with BCA assay (Bio-Rad). .. Cell lysates (~20 μg of protein per replicate) were transferred to 0.5 ml of LoBind deep-well plate (Eppendorf) prepared for mass spectrometry (MS) analysis using the following modified SP3 protocol ( ): Samples were subjected to simultaneous reduction and alkylation with a final concentration of 10 mM tris (2-carboxyethyl)phosphine and 40 mM 2-chloracetamide, followed by heating at 95°C for 10 min. Prewashed magnetic PureCube Carboxy agarose beads (20 μl; Cube Biotech) were added to all samples along with acetonitrile [ACN; 70% (v/v) final concentration] and incubated at room temperature for 20 min. ..

    other:

    Article Title: GID/CTLH E3 ligase complex control cell fate programs for sexual development of Plasmodium falciparum.
    Article Snippet: Samples were run with 150 paired-end sequencing on the Miniseq platform (Illumina).

    Article Title: Improving the Sensitivity of Protein Quantification by Immunoaffinity Liquid Chromatography─Triple Quadrupole Mass Spectrometry Using an Iterative Transition Summing Technique.
    Article Snippet: 80μL of final sample volume was transferred to an Eppendorf LoBind deep well plate for further preparation.

    Article Title: GID/CTLH E3 ligase complex control cell fate programs for sexual development of Plasmodium falciparum
    Article Snippet: Samples were run with 150 paired-end sequencing on the Miniseq platform (Illumina).

    Article Title: Functional characterisation of components in two Plasmodium falciparum Cullin-RING-Ligase complexes.
    Article Snippet: Protein concentration was determined using Pierce BCA Protein Assay Kit following manufacturers’ instructions.

    Modification:

    Article Title: Functional characterisation of components in two Plasmodium falciparum Cullin-RING-Ligase complexes
    Article Snippet: .. Cell lysates (20 μg protein per replicate) were transferred to 0.5 ml LoBind Deep Well plate (Eppendorf) prepared for mass spectrometry analysis using the modified SP3 protocol with some modifications. .. Briefly, samples were subjected to simultaneous reduction and alkylation with a final concentration of 10 mM Tris (2-carboxyethyl) phosphine (TCEP) and 40 mM 2-chloracetamide followed by heating at 95 °C for 10 min. Prewashed magnetic PureCube Carboxy agarose beads (20 μl, Cube Biotech) were added to all the samples along with acetonitrile (ACN,70% v/v final concentration) and incubated at room temperature for 20 min.

    Article Title: Caspase-2 deficiency drives pathogenic liver polyploidy and increases age-associated hepatocellular carcinoma in mice
    Article Snippet: Tissue lysates were sonicated and cleared by centrifugation at 20,000 g (4°C), and protein concentration was determined with BCA assay (Bio-Rad). .. Cell lysates (~20 μg of protein per replicate) were transferred to 0.5 ml of LoBind deep-well plate (Eppendorf) prepared for mass spectrometry (MS) analysis using the following modified SP3 protocol ( ): Samples were subjected to simultaneous reduction and alkylation with a final concentration of 10 mM tris (2-carboxyethyl)phosphine and 40 mM 2-chloracetamide, followed by heating at 95°C for 10 min. Prewashed magnetic PureCube Carboxy agarose beads (20 μl; Cube Biotech) were added to all samples along with acetonitrile [ACN; 70% (v/v) final concentration] and incubated at room temperature for 20 min. ..

    Analysis:

    Article Title: Caspase-2 deficiency drives pathogenic liver polyploidy and increases age-associated hepatocellular carcinoma in mice
    Article Snippet: Tissue lysates were sonicated and cleared by centrifugation at 20,000 g (4°C), and protein concentration was determined with BCA assay (Bio-Rad). .. Cell lysates (~20 μg of protein per replicate) were transferred to 0.5 ml of LoBind deep-well plate (Eppendorf) prepared for mass spectrometry (MS) analysis using the following modified SP3 protocol ( ): Samples were subjected to simultaneous reduction and alkylation with a final concentration of 10 mM tris (2-carboxyethyl)phosphine and 40 mM 2-chloracetamide, followed by heating at 95°C for 10 min. Prewashed magnetic PureCube Carboxy agarose beads (20 μl; Cube Biotech) were added to all samples along with acetonitrile [ACN; 70% (v/v) final concentration] and incubated at room temperature for 20 min. ..

    Concentration Assay:

    Article Title: Caspase-2 deficiency drives pathogenic liver polyploidy and increases age-associated hepatocellular carcinoma in mice
    Article Snippet: Tissue lysates were sonicated and cleared by centrifugation at 20,000 g (4°C), and protein concentration was determined with BCA assay (Bio-Rad). .. Cell lysates (~20 μg of protein per replicate) were transferred to 0.5 ml of LoBind deep-well plate (Eppendorf) prepared for mass spectrometry (MS) analysis using the following modified SP3 protocol ( ): Samples were subjected to simultaneous reduction and alkylation with a final concentration of 10 mM tris (2-carboxyethyl)phosphine and 40 mM 2-chloracetamide, followed by heating at 95°C for 10 min. Prewashed magnetic PureCube Carboxy agarose beads (20 μl; Cube Biotech) were added to all samples along with acetonitrile [ACN; 70% (v/v) final concentration] and incubated at room temperature for 20 min. ..

    Incubation:

    Article Title: Caspase-2 deficiency drives pathogenic liver polyploidy and increases age-associated hepatocellular carcinoma in mice
    Article Snippet: Tissue lysates were sonicated and cleared by centrifugation at 20,000 g (4°C), and protein concentration was determined with BCA assay (Bio-Rad). .. Cell lysates (~20 μg of protein per replicate) were transferred to 0.5 ml of LoBind deep-well plate (Eppendorf) prepared for mass spectrometry (MS) analysis using the following modified SP3 protocol ( ): Samples were subjected to simultaneous reduction and alkylation with a final concentration of 10 mM tris (2-carboxyethyl)phosphine and 40 mM 2-chloracetamide, followed by heating at 95°C for 10 min. Prewashed magnetic PureCube Carboxy agarose beads (20 μl; Cube Biotech) were added to all samples along with acetonitrile [ACN; 70% (v/v) final concentration] and incubated at room temperature for 20 min. ..



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